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human hct116 p53 proficient  (ATCC)


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    ATCC human hct116 p53 proficient
    Analysis of the selected senescence markers and anillin levels in <t>HCT116</t> <t>p53WT</t> and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001
    Human Hct116 P53 Proficient, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hct116+p53+proficient/pmc13061550-50-1-23?v=ATCC
    Average 99 stars, based on 4071 article reviews
    human hct116 p53 proficient - by Bioz Stars, 2026-08
    99/100 stars

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    1) Product Images from "Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence"

    Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

    Journal: Aging and Disease

    doi: 10.14336/AD.2025.0402

    Analysis of the selected senescence markers and anillin levels in HCT116 p53WT and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001
    Figure Legend Snippet: Analysis of the selected senescence markers and anillin levels in HCT116 p53WT and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

    Techniques Used: One-tailed Test, Expressing, Control, Western Blot, Fluorescence, Staining

    Analysis of selected senescence markers and anillin levels in HCT 116 p53KO cells induced to senescence by doxorubicin treatment for 1 day and collected 5 days after senescence induction. ( A ) Densitometric analysis of protein levels in control and doxorubicin-treated HCT116 p53KO based on Western blotting results, n=8; statistical analysis was performed using paired one-tailed t-Student test. Statistical significance is shown relative to control. ( B ) Representative images from Western blotting. ( C ) Comparison of anillin and p53 levels in p53-proficient (HCT116 p53WT and MCF7) and p53-deficient (HCT116 p53KO) cells treated with doxorubicin and analyzed using Western blotting, densitometric analysis (normalized to the level of anillin or p53 in control cells), n=3; statistical analysis was performed using Kruskal-Wallis test. Statistical significance is shown for differences between indicated cell lines. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. ( D ) Representative images of immunostained control and doxorubicin-treated HCT116 p53KO cells. Red – anillin, green – lamin A/C, blue – DAPI stained DNA. Scale 20 µm. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. Statistical significance: **** p ≤ 0.0001
    Figure Legend Snippet: Analysis of selected senescence markers and anillin levels in HCT 116 p53KO cells induced to senescence by doxorubicin treatment for 1 day and collected 5 days after senescence induction. ( A ) Densitometric analysis of protein levels in control and doxorubicin-treated HCT116 p53KO based on Western blotting results, n=8; statistical analysis was performed using paired one-tailed t-Student test. Statistical significance is shown relative to control. ( B ) Representative images from Western blotting. ( C ) Comparison of anillin and p53 levels in p53-proficient (HCT116 p53WT and MCF7) and p53-deficient (HCT116 p53KO) cells treated with doxorubicin and analyzed using Western blotting, densitometric analysis (normalized to the level of anillin or p53 in control cells), n=3; statistical analysis was performed using Kruskal-Wallis test. Statistical significance is shown for differences between indicated cell lines. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. ( D ) Representative images of immunostained control and doxorubicin-treated HCT116 p53KO cells. Red – anillin, green – lamin A/C, blue – DAPI stained DNA. Scale 20 µm. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. Statistical significance: **** p ≤ 0.0001

    Techniques Used: Control, Western Blot, One-tailed Test, Comparison, Expressing, Staining

    Correlation between p53 and anillin levels during senescence and the escape from senescence in breast cancer MCF-7 and colon cancer HCT116 p53WT cells (see ). ( A-B ). Representative Western blots showing the levels of anillin and p53 in HCT116 p53WT cells ( A ) and MCF-7 cells ( B ). ( C-F ) The level of anillin and p53 on subsequent days of cell culture after senescence induction by doxorubicin in HCT116 p53WT ( C and E ) and MCF-7 cells ( D and F ) n = 4; statistical analysis was performed using one-way ANOVA followed by post hoc analysis (Tukey’s honest significant difference test; HSD test). Statistical significance of differences between indicated days of treatment: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( G ) The heat map shows the levels of anillin and p53 during senescence and escape from senescence in MCF-7 and HCT116 p53WT cells. Heatmap: Original data points are standardized into z-scores.
    Figure Legend Snippet: Correlation between p53 and anillin levels during senescence and the escape from senescence in breast cancer MCF-7 and colon cancer HCT116 p53WT cells (see ). ( A-B ). Representative Western blots showing the levels of anillin and p53 in HCT116 p53WT cells ( A ) and MCF-7 cells ( B ). ( C-F ) The level of anillin and p53 on subsequent days of cell culture after senescence induction by doxorubicin in HCT116 p53WT ( C and E ) and MCF-7 cells ( D and F ) n = 4; statistical analysis was performed using one-way ANOVA followed by post hoc analysis (Tukey’s honest significant difference test; HSD test). Statistical significance of differences between indicated days of treatment: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( G ) The heat map shows the levels of anillin and p53 during senescence and escape from senescence in MCF-7 and HCT116 p53WT cells. Heatmap: Original data points are standardized into z-scores.

    Techniques Used: Western Blot, Cell Culture

    Inverse correlation between ANLN and p53 during senescence and escape from senescence in cancer cells. Downregulation of anillin is a consequence of the induction of p53 due to cellular senescence. After some time, senescent cells resume divisions, which is associated with a decrease in p53 levels and an increase in anillin. Performed with Biorender.
    Figure Legend Snippet: Inverse correlation between ANLN and p53 during senescence and escape from senescence in cancer cells. Downregulation of anillin is a consequence of the induction of p53 due to cellular senescence. After some time, senescent cells resume divisions, which is associated with a decrease in p53 levels and an increase in anillin. Performed with Biorender.

    Techniques Used:



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    human hct116 p53 proficient  (ATCC)
    99
    ATCC human hct116 p53 proficient
    Analysis of the selected senescence markers and anillin levels in <t>HCT116</t> <t>p53WT</t> and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001
    Human Hct116 P53 Proficient, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hct116+p53+proficient/pmc13061550-50-1-23?v=ATCC
    Average 99 stars, based on 1 article reviews
    human hct116 p53 proficient - by Bioz Stars, 2026-08
    99/100 stars
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    Analysis of the selected senescence markers and anillin levels in HCT116 p53WT and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

    Journal: Aging and Disease

    Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

    doi: 10.14336/AD.2025.0402

    Figure Lengend Snippet: Analysis of the selected senescence markers and anillin levels in HCT116 p53WT and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

    Article Snippet: The human HCT116 p53-proficient (referred to as HCT116 p53WT) colon cancer cell line and the breast cancer cell line MCF-7 were obtained from ATCC (HCT116 CCL-247; MCF-7 HTB-22).

    Techniques: One-tailed Test, Expressing, Control, Western Blot, Fluorescence, Staining

    Analysis of selected senescence markers and anillin levels in HCT 116 p53KO cells induced to senescence by doxorubicin treatment for 1 day and collected 5 days after senescence induction. ( A ) Densitometric analysis of protein levels in control and doxorubicin-treated HCT116 p53KO based on Western blotting results, n=8; statistical analysis was performed using paired one-tailed t-Student test. Statistical significance is shown relative to control. ( B ) Representative images from Western blotting. ( C ) Comparison of anillin and p53 levels in p53-proficient (HCT116 p53WT and MCF7) and p53-deficient (HCT116 p53KO) cells treated with doxorubicin and analyzed using Western blotting, densitometric analysis (normalized to the level of anillin or p53 in control cells), n=3; statistical analysis was performed using Kruskal-Wallis test. Statistical significance is shown for differences between indicated cell lines. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. ( D ) Representative images of immunostained control and doxorubicin-treated HCT116 p53KO cells. Red – anillin, green – lamin A/C, blue – DAPI stained DNA. Scale 20 µm. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. Statistical significance: **** p ≤ 0.0001

    Journal: Aging and Disease

    Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

    doi: 10.14336/AD.2025.0402

    Figure Lengend Snippet: Analysis of selected senescence markers and anillin levels in HCT 116 p53KO cells induced to senescence by doxorubicin treatment for 1 day and collected 5 days after senescence induction. ( A ) Densitometric analysis of protein levels in control and doxorubicin-treated HCT116 p53KO based on Western blotting results, n=8; statistical analysis was performed using paired one-tailed t-Student test. Statistical significance is shown relative to control. ( B ) Representative images from Western blotting. ( C ) Comparison of anillin and p53 levels in p53-proficient (HCT116 p53WT and MCF7) and p53-deficient (HCT116 p53KO) cells treated with doxorubicin and analyzed using Western blotting, densitometric analysis (normalized to the level of anillin or p53 in control cells), n=3; statistical analysis was performed using Kruskal-Wallis test. Statistical significance is shown for differences between indicated cell lines. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. ( D ) Representative images of immunostained control and doxorubicin-treated HCT116 p53KO cells. Red – anillin, green – lamin A/C, blue – DAPI stained DNA. Scale 20 µm. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. Statistical significance: **** p ≤ 0.0001

    Article Snippet: The human HCT116 p53-proficient (referred to as HCT116 p53WT) colon cancer cell line and the breast cancer cell line MCF-7 were obtained from ATCC (HCT116 CCL-247; MCF-7 HTB-22).

    Techniques: Control, Western Blot, One-tailed Test, Comparison, Expressing, Staining

    Correlation between p53 and anillin levels during senescence and the escape from senescence in breast cancer MCF-7 and colon cancer HCT116 p53WT cells (see ). ( A-B ). Representative Western blots showing the levels of anillin and p53 in HCT116 p53WT cells ( A ) and MCF-7 cells ( B ). ( C-F ) The level of anillin and p53 on subsequent days of cell culture after senescence induction by doxorubicin in HCT116 p53WT ( C and E ) and MCF-7 cells ( D and F ) n = 4; statistical analysis was performed using one-way ANOVA followed by post hoc analysis (Tukey’s honest significant difference test; HSD test). Statistical significance of differences between indicated days of treatment: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( G ) The heat map shows the levels of anillin and p53 during senescence and escape from senescence in MCF-7 and HCT116 p53WT cells. Heatmap: Original data points are standardized into z-scores.

    Journal: Aging and Disease

    Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

    doi: 10.14336/AD.2025.0402

    Figure Lengend Snippet: Correlation between p53 and anillin levels during senescence and the escape from senescence in breast cancer MCF-7 and colon cancer HCT116 p53WT cells (see ). ( A-B ). Representative Western blots showing the levels of anillin and p53 in HCT116 p53WT cells ( A ) and MCF-7 cells ( B ). ( C-F ) The level of anillin and p53 on subsequent days of cell culture after senescence induction by doxorubicin in HCT116 p53WT ( C and E ) and MCF-7 cells ( D and F ) n = 4; statistical analysis was performed using one-way ANOVA followed by post hoc analysis (Tukey’s honest significant difference test; HSD test). Statistical significance of differences between indicated days of treatment: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( G ) The heat map shows the levels of anillin and p53 during senescence and escape from senescence in MCF-7 and HCT116 p53WT cells. Heatmap: Original data points are standardized into z-scores.

    Article Snippet: The human HCT116 p53-proficient (referred to as HCT116 p53WT) colon cancer cell line and the breast cancer cell line MCF-7 were obtained from ATCC (HCT116 CCL-247; MCF-7 HTB-22).

    Techniques: Western Blot, Cell Culture

    Inverse correlation between ANLN and p53 during senescence and escape from senescence in cancer cells. Downregulation of anillin is a consequence of the induction of p53 due to cellular senescence. After some time, senescent cells resume divisions, which is associated with a decrease in p53 levels and an increase in anillin. Performed with Biorender.

    Journal: Aging and Disease

    Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

    doi: 10.14336/AD.2025.0402

    Figure Lengend Snippet: Inverse correlation between ANLN and p53 during senescence and escape from senescence in cancer cells. Downregulation of anillin is a consequence of the induction of p53 due to cellular senescence. After some time, senescent cells resume divisions, which is associated with a decrease in p53 levels and an increase in anillin. Performed with Biorender.

    Article Snippet: The human HCT116 p53-proficient (referred to as HCT116 p53WT) colon cancer cell line and the breast cancer cell line MCF-7 were obtained from ATCC (HCT116 CCL-247; MCF-7 HTB-22).

    Techniques: